Coding

Part:BBa_K3944027:Design

Designed by: Olof Dahlman   Group: iGEM21_Chalmers-Gothenburg   (2021-09-30)


VP16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence has been optimised for use in S.Cerevisiae. Sequence originates from a risk class 3 organism, but posses no pathogenic activity on its own and has been well-characterised for use in fusion proteins (1).


Source

Provided by systems biology department at Chalmers technical highschool.

References

1: Hirai H, Tani T, Kikyo N. Structure and functions of powerful transactivators: VP16, MyoD and FoxA. Int J Dev Biol. 2010;54(11-12):1589-1596. doi:10.1387/ijdb.103194hh