Regulatory

Part:BBa_K3944024:Design

Designed by: Olof Dahlman   Group: iGEM21_Chalmers-Gothenburg   (2021-09-30)


ScGAL1 - Z3BS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 169
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promoter and recognition sequence designed for use with beta-estradiol system (1).


Source

Provided by systems biology department at Chalmers technical highschool.

References

1: R. Scott McIsaac, Patrick A. Gibney, Sunil S. Chandran, Kirsten R. Benjamin, David Botstein, Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae, Nucleic Acids Research, Volume 42, Issue 6, 1 April 2014, Page e48, https://doi.org/10.1093/nar/gkt1402