Regulatory

Part:BBa_K3944000:Design

Designed by: Olof Dahlman   Group: iGEM21_Chalmers-Gothenburg   (2021-09-14)


ScCUP1p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The system is native to yeast and the only consideration is to not remove the native parts, lest induction via copper results in high cellular toxicity and cell death.


Source

Retrieved from pYTK031, plasmid bearing component, part of Lee et.al (2015)(1) MoClo library kit.

References

1: A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly. Lee ME, DeLoache WC, Cervantes B, Dueber JE. ACS Synth Biol. 2015 Apr 14. 10.1021/sb500366v PubMed 25871405