Regulatory

Part:BBa_K3942003:Design

Designed by: Igor Blatov   Group: iGEM21_MIPT_MSU   (2021-10-13)


IRES


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If IRES-GFP cassette is used, it may interfere with translation of smaller size cDNAs (like SLA) or generate fusion proteins and entail defective virus production in an unpredictable manner.


Source

The part was taken from the plasmid pCDH-ZEB1-Flag-HA-puro provided by the Laboratory of Cell Signalling Regulation.

References

Encarnacion Martinez-Salas*, Rosario Francisco-Velilla, Javier Fernandez-Chamorro and Azman M. Embarek "Insights into Structural and Mechanistic Features of Viral IRES Elements" Front. Microbiol., 04 January 2018 | https://doi.org/10.3389/fmicb.2017.02629