Coding

Part:BBa_K3941000:Design

Designed by: Begüm Esra Aytan   Group: iGEM21_Saint_Joseph   (2021-07-26)


CelAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is created from only the catalytic cellulase region of Teredinibacter turnerae T8602 celAB protein by excluding any other binding site/domain residues. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag is added to 3' end of the sequence to help purification of protein product.


Source

This part is created based on Teredinibacter turnerae T8602 celAB (endo-1, 4-β-D glucanase) protein and this enzyme degrades wood lignocellulose in nature.

References