Device

Part:BBa_K3937011:Design

Designed by: Xuanshuo Zhang   Group: iGEM21_NPU-CHINA   (2021-10-01)


BADE+Lysis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 497
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1040
    Illegal BamHI site found at 82
    Illegal XhoI site found at 935
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 497
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 497
    Illegal AgeI site found at 254
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We integrade Lac operon and BADE enzyme and our lysis system.


Source

From Bacillus subtilis 168 genome and being assembled.

References