Coding

Part:BBa_K3930024:Design

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)


LCYe-ofCCD1m fusion with a LGS linker to produce α-ionone in Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 282
    Illegal BglII site found at 812
    Illegal BamHI site found at 396
    Illegal BamHI site found at 2535
    Illegal BamHI site found at 3387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

he LcyE sequence comes from Latuca sativa and ofCCD1 comes from Osmanthus fragrans and the fusion has been synthetized by IDT. The idea comes from the article (Chen et al. 2019).

References