Other

Part:BBa_K3930013:Design

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)


Down integrative sequence to target locus X-3 of S. cerevisiae genome


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 71
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 71
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 71
    Illegal XhoI site found at 378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 71
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The sequence come from the plasmid pCfB3034 of the EasyClone-MarkerFree kit (Jessop-Fabre et al.,2016)

References