Coding

Part:BBa_K3930000:Design

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-09-20)


Linalool and dihydro-β-ionone induction system and expression in S. cerevisiae (pFLEUR)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1568
    Illegal XbaI site found at 1871
    Illegal XbaI site found at 6117
    Illegal XbaI site found at 6477
    Illegal SpeI site found at 4363
    Illegal SpeI site found at 8464
    Illegal PstI site found at 256
    Illegal PstI site found at 1213
    Illegal PstI site found at 1384
    Illegal PstI site found at 1887
    Illegal PstI site found at 3251
    Illegal PstI site found at 7610
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6555
    Illegal SpeI site found at 4363
    Illegal SpeI site found at 8464
    Illegal PstI site found at 256
    Illegal PstI site found at 1213
    Illegal PstI site found at 1384
    Illegal PstI site found at 1887
    Illegal PstI site found at 3251
    Illegal PstI site found at 7610
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 293
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1568
    Illegal XbaI site found at 1871
    Illegal XbaI site found at 6117
    Illegal XbaI site found at 6477
    Illegal SpeI site found at 4363
    Illegal SpeI site found at 8464
    Illegal PstI site found at 256
    Illegal PstI site found at 1213
    Illegal PstI site found at 1384
    Illegal PstI site found at 1887
    Illegal PstI site found at 3251
    Illegal PstI site found at 7610
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1568
    Illegal XbaI site found at 1871
    Illegal XbaI site found at 6117
    Illegal XbaI site found at 6477
    Illegal SpeI site found at 4363
    Illegal SpeI site found at 8464
    Illegal PstI site found at 256
    Illegal PstI site found at 1213
    Illegal PstI site found at 1384
    Illegal PstI site found at 1887
    Illegal PstI site found at 3251
    Illegal PstI site found at 7610
    Illegal AgeI site found at 2074
    Illegal AgeI site found at 2840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2396
    Illegal BsaI site found at 6658
    Illegal BsaI.rc site found at 1534
    Illegal SapI site found at 8626


Design Notes

PCR, In Fusion and integration into yeast


Source

genomic, codon optimized

References