Regulatory
Pyear-1

Part:BBa_K3926002:Design

Designed by: Chen Kening   Group: iGEM21_XHD-Wuhan-A-China   (2021-10-01)


An improved PyeaR with higher expression strength


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use machine learning model to design this promoter.

Source

The original sequence is from BBa_K216005.

References

Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in Escherichia coli K-12. J. Bacteriol. 189, 7539-7548