Device

Part:BBa_K392044

Designed by: Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi   Group: iGEM10_Osaka   (2010-10-25)


beta-glucosidase/CBM secretion testing construct for E. coli

Device containing C. fermi beta-glucosidase fused with cellulose-binding motif CM10; E. coli PelB export tag attached upstream to enable secretion of protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1374
    Illegal XhoI site found at 1607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 863
    Illegal AgeI site found at 306
    Illegal AgeI site found at 365
    Illegal AgeI site found at 759
    Illegal AgeI site found at 954
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 736


[edit]
Categories
Parameters
None