Device

Part:BBa_K392043:Design

Designed by: Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi   Group: iGEM10_Osaka   (2010-10-25)


beta-glucosidase secretion testing construct for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1374
    Illegal XhoI site found at 1607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 863
    Illegal AgeI site found at 306
    Illegal AgeI site found at 365
    Illegal AgeI site found at 759
    Illegal AgeI site found at 954
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 736


Design Notes

Assembly included Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead.


Source

Assembly of R0010, B0034, J32015, K392008, B0010.

References