Device

Part:BBa_K392040:Design

Designed by: Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi   Group: iGEM10_Osaka   (2010-10-25)


endoglucanase/CBM secretion testing construct for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 331
    Illegal NotI site found at 1475
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1388
    Illegal BamHI site found at 524
    Illegal XhoI site found at 886
    Illegal XhoI site found at 1135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 666
    Illegal NgoMIV site found at 1591
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 570


Design Notes

Assembly included Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead.


Source

Assembly of R0010, B0034, J32015, K392006, K392014, B0010.

References