Coding

Part:BBa_K3909001

Designed by: Xixi Song   Group: iGEM21_Worldshaper-Nanjing   (2021-10-14)


ylPOX2

This part, ylPOX2 , is one of candidate acyl-CoA oxidase genes involved in fatty acid degradation experiments.

Our goal is to improve the cell growth of Y. lipolytica and convert a large amount of gutter oil to γ-linolenic acid when Y. lipolytica grows with gutter oil as the sole carbon source . We plan to enhance the oli degradation pathway by expressing three endogenous fatty acid degradation genes ylMEF1 (BBa_K3909006), ylPOT1 (BBa_K3909007), and ylPOXn (from BBa_K3909000 to BBa_K3909005), which are related to the metabolim of transforming acyl-CoA into acetyl-CoA in peroxisome (β-oxidation). Specifically, the β-oxidation includes three steps: i) oxidation, that catalyzed by six acyl-CoA oxidases (translated from ylPOX1 to ylPOX6); ii) hydration and dehydration, that catalyzed by multifunctional enzyme (translated from ylMFE1); and iii) thiolysis, that catalyzed by 3-ketoacyl-CoA thiolase (translated from ylPOT1)[1].

References

[1] Green A , Silver P , Collins J , et al. Toehold switches: de-novo-designed regulators of gene expression.[J]. Cell, 2014, 159(4):925-939.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 785
    Illegal XhoI site found at 517
    Illegal XhoI site found at 1819
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1171
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 514
    Illegal BsaI site found at 1225
    Illegal BsaI.rc site found at 188
    Illegal BsaI.rc site found at 449
    Illegal BsaI.rc site found at 520
    Illegal BsaI.rc site found at 544
    Illegal BsaI.rc site found at 1021
    Illegal BsaI.rc site found at 2009
    Illegal SapI site found at 1206


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