RNA
Part:BBa_K3907003
Designed by: Laura Keffer-Wilkes Group: iGEM21_Lethbridge_HS (2021-10-12)
siRNA_scramble against Clpp
This construct contains 26 repeating copies of a 19 bp fragment that is a scrambled version of BBa_K3907002 against the Arabidopsis gene ClpP. The scramble sequence can be used as a control to test the efficacy of the original siRNA construct. The DNA is to be used as a template to create siRNA fragments using RNase H and DNA chimer splints as per Feyrer et al. (2020).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 330
Illegal PstI site found at 344 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 330
Illegal PstI site found at 344
Illegal NotI site found at 337 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 330
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 330
Illegal PstI site found at 344 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |