Composite
Part:BBa_K3906000:Design
Designed by: Ruyi Shi Group: iGEM21_ASTWS-China (2021-10-14)
PETase-SpyCatcher
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 195
Design Notes
For strong PETase expression, PETase was inserted into the pET-21a(+) vector and we did codon optimization for successful E. coli expression. In order to make PETase and MHETase effectively coupled, SypCatcher and SpyTag system was used.
Source
The sequence was based on BBa_K3576001.