Composite

Part:BBa_K3905011

Designed by: Peter Heywood, Harry Weiniger   Group: iGEM21_City_of_London_UK   (2021-09-13)


T7 / gen3 210-3p-517-5p AND-Gate Anti-miRNA / Terminator

This composite part synthesises an anti-miRNA that is vital to the use of our AND gate toehold switch (part BBa_K3905006). This anti-miRNA is an RNA strand containing bases complementary to both the 517-5p and 210-3p micro-RNAs. This means that when both RNAs are present a complex can form that can be used as a trigger for our AND toehold switch. It is preceded by a T7 promoter for functional RNAs (Part:BBa_K1614000) which is best suited for our coded region.


Diagram of Trigger Complex formation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None