RNA

Part:BBa_K3905003

Designed by: Peter Heywood, Harry Weiniger   Group: iGEM21_City_of_London_UK   (2021-09-05)


gen1 210-3p Anti-miRNA

Our Anti-miRNA has two halves: The first half of this RNA strand binds to half of miRNA hsa210-3p whilst the second half consists of multiple repeated Guanine bases. This creates a duplex with a trigger region of the anti-miRNA and the miRNA 210-3p. This trigger can turn BBA_K3905002 (gen1 210-3p Toehold Switch) into its 'on' state.

Diagram of antiRNA and miRNA binding.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None