Part:BBa_K3904202
loop_BBa1033225_GFP
To assure continuous and efficient naringenin production, we had to guarantee an effective expression of naringenin synthesis enzymes in our probiotic strains. To mimic the natural synthesis pathway, we decided to manipulate the expression rates of these proteins by finding the promoters of optimal strength for the expression of each of these enzymes.
The strength of promoters was estimated by measuring of how intensively the nissle transformants can produce GFP under the promoter of interest. The intensity of fluorescence was evaluated by dividing the intensity of the signal by the OD600 of the medium in 6 hours. This way the sronger fluorescence signal would indicate the stronger promoter.
The data was compared between all the promoters of our interest and the sequences demonstrating required expression rates were selected for the construction of naringenin synthesis cassete.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 882
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 177
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