Coding

Part:BBa_K3898163:Design

Designed by: Yuliang Huo   Group: iGEM21_DUT_China   (2021-10-01)


RIDD-MHETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal PstI site found at 968
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 968
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1308
    Illegal XhoI site found at 1958
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal PstI site found at 968
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal PstI site found at 968
    Illegal NgoMIV site found at 193
    Illegal AgeI site found at 1006
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was optimized for E. coli BL21 using the Codon Optimization Tool from the genscript website.

Source

BBa_K3898001, BBa_K3898006

References