Coding
Part:BBa_K3898163:Design
Designed by: Yuliang Huo Group: iGEM21_DUT_China (2021-10-01)
RIDD-MHETase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
Illegal PstI site found at 968 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 968
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1308
Illegal XhoI site found at 1958 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
Illegal PstI site found at 968 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
Illegal PstI site found at 968
Illegal NgoMIV site found at 193
Illegal AgeI site found at 1006 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was optimized for E. coli BL21 using the Codon Optimization Tool from the genscript website.
Source
BBa_K3898001, BBa_K3898006