Protein_Domain

Part:BBa_K3896011

Designed by: Zihan Wu   Group: iGEM21_NEU_CHINA   (2021-10-01)


SARS-CoV-2 RBD with His-tag

The target DNA can express a protein containing 194 amino acids, which is a segment of SARS-CoV-2 RBD, including Thr333 to Gly526. It is a key part of binding to receptor ACE2. The C-terminal has his tag, which is convenient for subsequent protein extraction and purification. The purified protein can specifically bind to pmrCAB (ACE2) to trigger downstream reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 484
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 484
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 610
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 484
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 484
    Illegal NgoMIV site found at 433
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None