Part:BBa_K389403:Design

Sensitivity tuner + luciferase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1322
  Illegal NgoMIV site found at 2666
  Illegal NgoMIV site found at 2687
  Illegal AgeI site found at 845
  Illegal AgeI site found at 957
  Illegal AgeI site found at 1159
  Illegal AgeI site found at 2390
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 2572
  

Design Notes

arabinose promoter removed, GFP read-out exchanged with luciferase compared to the original part BBa_I746390

Source

• luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
• sensitivity tuner part consists inter alia of
  • delta activator from phage phiR73 (BBa_I746352)
  • PF promoter from phage P2 (BBa_I746360)

References

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]