Reporter

Part:BBa_K389403:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)

Sensitivity tuner + luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 2666
    Illegal NgoMIV site found at 2687
    Illegal AgeI site found at 845
    Illegal AgeI site found at 957
    Illegal AgeI site found at 1159
    Illegal AgeI site found at 2390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2572


Design Notes

arabinose promoter removed, GFP read-out exchanged with luciferase compared to the original part BBa_I746390

Source

  • luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
  • sensitivity tuner part consists inter alia of

References

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]