Coding

Part:BBa_K3894017

Designed by: Jiaqi Zhang   Group: iGEM21_NEFU_China   (2021-10-17)


BbvCI-R1
BbvC R1 is one of two heterogeneous subunits of the R.BbvCI enzyme.

Description

BbvCI-R1 is one of two heterogeneous subunits of the R.BbvCI enzyme. The recognition sequence of the non-palindrome restriction endonuclease BbvCI from Bacillus brevis is CC^TCAGC/GC^TGAGG (^ indicating cleavage site). Its R1 subunit cleaves the bottom strand GC^TGAGG. This subunit contains a catalytic site for DNA hydrolysis and works independently with strand specificity. The Nb.BbvCI is a restriction enzyme that cleaves duplex DNA within a seven base pair asymmetric recognition sequence, and this activity depends on the combinatorial usage of the two subunits.[1]

References

[1] DF Heiter, Lunnen K D , Wilson G G . Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI.[J]. Journal of Molecular Biology, 2005, 348(3):631-640.

Sequence and Features Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None