Composite

Part:BBa_K389302:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-12)

weak constitutive promoter + luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 125
    Illegal NgoMIV site found at 1469
    Illegal NgoMIV site found at 1490
    Illegal AgeI site found at 1193
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1375


Design Notes

weak promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working

Source

  • promoter and RBS from parts.igem
  • luciferase from pGL4.10[luc2] (Promega)

References