Terminator

Part:BBa_K3893006:Design

Designed by: Vanessa Gaona   Group: iGEM21_Ecuador   (2021-10-20)


rpoC Transcriptional terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure that the part is suitable with the assembly RFC10. Therefore, the sequence does not have restriction recognition sites for the enzymes: EcoRI, XbaI, SpeI, PstI and NotI

Source

It comes from a genetic reported sequence

References

Cambray, G., Guimaraes, J. C., Mutalik, V. K., Lam, C., Mai, Q. A., Thimmaiah, T., ... & Endy, D. (2013). Measurement and modeling of intrinsic transcription terminators. Nucleic acids research, 41(9), 5139-5148.