Coding

Part:BBa_K3893005:Design

Designed by: Camila Gallegos   Group: iGEM21_Ecuador   (2021-10-20)


Lysozyme C (Lysis protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 434
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure that the part is suitable with the assembly RFC10. Therefore, the sequence does not have restriction recognition sites for the enzymes: EcoRI, XbaI, SpeI, PstI and NotI


Source

It comes from a genetic reported sequence

References

Borrero-de Acuña, J. M., Hidalgo-Dumont, C., Pacheco, N., Cabrera, A., & Poblete-Castro, I. (2017). A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production. Scientific reports, 7(1), 1-11.