Coding
Link-GFP

Part:BBa_K3892005:Design

Designed by: iGEM21_Linkoping_Sweden   Group: iGEM21_Linkoping_Sweden   (2021-10-16)


Linker-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 338
    Illegal XhoI site found at 676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 40
    Illegal AgeI site found at 174
    Illegal AgeI site found at 588
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 659


Design Notes

A short glycine linker was added to the start of the sequence as well.


Source

Aequorea victoria

References