Part:BBa_K389052:Experience

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User Reviews

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Jonas Aretz

This construct was plated for plasmid isolation after assembly - and I have never seen such red plates when working with constructs with mRFP downstream of a promoter. This lac operon definitely shows a very high basal transcription, so it is not tightly repressed. It seems that the lac repressor BBa_C0012 is not suitable for this purpose due to its degradation tag or it does not work properly.

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