Regulatory

Part:BBa_K3890001:Design

Designed by: Beatriz Toledo Akiti   Group: iGEM21_USP-Brazil   (2021-09-29)


pLAT52 pollen specific promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Eukaryote promoters can be very long. The sequence that was used was based off articles that specify the minimal sequence for pollen specific guided expression.


Source

NCBI Solanum lycopersicum genome Bate N, Twell D. Functional architecture of a late pollen promoter: pollen-specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elements. Plant Mol Biol. 1998 Jul;37(5):859-69. doi: 10.1023/a:1006095023050. PMID: 9678581. TWELL, D. & McCormick, S.. Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis. 1990 Jul;109(3):705-13. PMID: 2401221.


References