Composite
Part:BBa_K3890000:Design
Designed by: Beatriz Toledo Akiti Group: iGEM21_USP-Brazil (2021-09-30)
Pollen expressed CYP6G1 and GUS reporter circuit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2088
Illegal SapI site found at 1918
Design Notes
The CYP codons are optimized for tomato expression
Source
NCBI Solanum lycopersicum genome NCBI Drosophila melanogaster genome SUN H, Zhou N, Wang H, Huang D, Lang Z. Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system. PLoS One. 2017 Mar 30;12(3):e0174804. doi: 10.1371/journal.pone.0174804. PMID: 28358924; PMCID: PMC5373624.