DNA

Part:BBa_K3887013

Designed by: Ran Wang   Group: iGEM21_BJU_China   (2021-10-01)


sgRNA3

Single guide RNA sequence we designed for TnaA knockout in E.coli BL21(DE3).

Single guide RNA (sgRNA) is used during gene editing process. It is the sequences of RNA molecules that bind to the target location. It is made of crRNA and trcrRNA. During the editing process, tracrRNA first recognizes the Cas9 and binds to it. The Cas9 receives sequence information from the crRNA and moves to the target location to perform the cleaving of DNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None