Coding

Part:BBa_K3887000

Designed by: Ran Wang   Group: iGEM21_BJU_China   (2021-10-01)


Fre-L3

A soluble partner and cofactor supply for fusion enzyme.

Description

Fre is a flavin reductase that regenerates FADH2 and highly expressed as a soluble form in E. coli, L3 is a rigid linker commonly used for fusion enzymes.

In our experiment, Fre-L3 is used as a N-terminal tag and cofactor supply with three halogenases (SttH, PyrH, RebH) as strategy to enhance the solubility of halogenases in vivo. Thus, the fusion enzymes could compatible with whole-cell reactions or metabolic engineering. It is necessary to increase their activity as well as the yield of target molecules.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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