Composite

Part:BBa_K3886011

Designed by: Meng Fankang   Group: iGEM21_NDNF_China   (2021-10-01)


fimA target gRNA Expression

This is a part used in CRISPR-Cas9-mediated genome editing. gRNA is used to guide Cas9 protein to the target fimA genome site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None