Part:BBa_K3885221

J23119-gRNA

In the presence of a strong promoter J23119, the gRNA could be expressed.

Usage and Biology

In the presence of a strong promoter J23119,the gRNA could be expressed.In this scientific research project,the gRNA interacts with genetic circuits to achieve the goal of detecting specific biomarker.

Characterization

CRISPR-Cas9 is a powerful DNA editing tool.A gRNA directs Cas9 to cleave any DNA sequence with a PAM.The gRNA is an important part of the CRISPR system.We have engineered gRNA so that its secondary structure has been transformed.

References

[1]Moreb E A,Lynch M D. Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity.[J]. Nature communications,2021,12(1):
[2]Hassan Md Mahmudul,Zhang Yingxiao,Yuan Guoliang,De Kuntal,Chen JinGui,Muchero Wellington,Tuskan Gerald A,Qi Yiping,Yang Xiaohan. Construct design for CRISPR/Cas-based genome editing in plants.[J]. Trends in plant science,2021:
[3]He Chao,Liu Hao,Chen Dijun,Xie WenZhao,Wang Mengxin,Li Yuqi,Gong Xin,Yan Wenhao,Chen LingLing. CRISPR-Cereal: A Guide RNA Design Tool Integrating Regulome and Genomic Variation for Wheat, Maize and Rice.[J]. Plant biotechnology journal,2021:

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]