Composite
Part:BBa_K3883030:Design
Designed by: Liu Yuqi Group: iGEM21_Jilin_China (2021-10-20)
targeted knockdown the expression of ppc gene through CRISPRi
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1208
Illegal NheI site found at 4231
Illegal NheI site found at 4254 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 49
Design Notes
targeted knockdown the expression of ppc gene through CRISPRi to downregulate TCA cycle
Source
targeted knockdown the expression of ppc gene through CRISPRi to downregulate TCA cycle