Part:BBa_K3882001:Experience

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Applications of BBa_K3882001

E. bsuahlscout bio-brick can transform to any competent bacterial. We use E. Coli BL21 as a recipe and safe bacterial to do the transformation. We use the LB media to culture the E. bsuahlscout. When the OD value reach to 0.2-0.4, we add 200 uM IPTG into the LB media to activate the E. bsuahlscout bio-brick inside at 30 degree centigrade for 4 h. After that, we make the AHL detecting kit and 1 cube is from LB-agar plate which contains 500 uM IPTG. The sample LB media which contains AHL will culture the activated E. bsuahlscout at 30 about 1-2 hours until the red color show up. E. bsuahlteminator bio-brick is automatically activate by QS promotor including LUXR-AHL complex and Lux operator. The more AHL in the environment and the more PVDQ-GFP will be produced.

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