DNA

Part:BBa_K3878009:Design

Designed by: Mathis LAVAUD   Group: iGEM21_UParis_BME   (2021-09-27)


Toehold_miR141_3 (to Toehold_miR141_6)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 105
    Illegal SpeI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 105
    Illegal SpeI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 105
    Illegal SpeI site found at 141
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part can be used with molecular cloning into a plasmid coding for a repressor gene followed by a T7 terminator (BBa_K3878000).


Source

From IDT

References