RNA
mirna122

Part:BBa_K3876501:Design

Designed by: Kovacs Viktoria   Group: iGEM21_SZTA_RMG_Szeged   (2021-08-10)


mimic miRNA-122

Lambert iGEM 2022: hsa-miR-122-5p


Sequence of hsa-miR-122-5p: UGGAGUGUGACAAUGGUGUUUG

The sequence of hsa-miR-122-5p was taken from miRBase, and we chose the mature sequence as it is found in circulation. No changes were made as these were the target sequence.

References:

Mature sequence hsa-miR-122-5p. Mirbase. (n.d.). Retrieved October 10, 2022, from https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MIMAT0000421





Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This mimic RNA will be used as a target to detect in a mimic body fluid.


Source

miRBase: https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000442

References