Composite

Part:BBa_K3875030

Designed by: Yimiao Lin   Group: iGEM21_BUCT   (2021-10-05)


Usage and Biology

Phyb-mazE-T1

Biology:
Phyb :the promoter of the hyb operon
mazE :antitoxic gene


Design:
We connect MazE with the phyb promoter to ensure that the mazE protein is expressed only under anaerobic conditions, and once the engineered bacteria are exposed to oxygen, drastic changes in oxygen concentration will cause the phyb promoter expression to fail, thus preventing the expression of antitoxins, which in turn will lead to the death of the engineering bacteria


Reference:
Richard, D. J., Sawers, G., Sargent, F., McWalter, L., & Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology (Reading, England), 145 ( Pt 10), 2903–2912. https://doi.org/10.1099/00221287-145-10-2903

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None