Reporter
Part:BBa_K3873999:Design
Designed by: Qinwen Wang Group: iGEM21_NJMU-China (2021-10-04)
An AND gate composed of a 36℃-induced promoter and Tryptophan riboswitch as a multi input modu
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 569 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 854
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1157
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
Biotechnol Bioeng. 2018 Jan;115(1):266-271. doi: 10.1002/bit.26448. Epub 2017 Oct 6. Systematic optimization of L-tryptophan riboswitches for efficient monitoring of the metabolite in Escherichia coli