Reporter

Part:BBa_K3873999

Designed by: Qinwen Wang   Group: iGEM21_NJMU-China   (2021-10-04)


An AND gate composed of a 36℃-induced promoter and Tryptophan riboswitch as a multi input modu

We constructed the TlpA36/PtlpA36 system to guarantee that the expression of tryptone aptamer can only happen if the temperature is above 36 degree Celsius. As for the aptamer, we are inspired by 2018 Oxford who used a riboswitch for adenine so we referred to some articles and found the riboswitch of tryptophan. It consists of a tryptone aptamer and a ribosome.


OT-MODEL.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 854
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1157
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
chassisE.coli