Regulatory

Part:BBa_K387012:Design

Designed by: Han Zhu   Group: iGEM10_SJTU-BioX-Shanghai   (2010-10-15)


CRE-JeT promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 496
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 484
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We invited a BGH polyA sequence in the front of this promoter to attenuate the influence of constitutive promoters in other commercial vectors (because this promoter should be inserted into shuttle plasmids or virus plasmids).


Source

This promoter is synthesized de novo using DNA synthesis techniques

References