Regulatory

Part:BBa_K387011:Design

Designed by: Han Zhu   Group: iGEM10_SJTU-BioX-Shanghai   (2010-10-15)


MEF2-JeT promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 472
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 251
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We invited a BGH polyA sequence in the front of this promoter to attenuate the influence of constitutive promoters in other commercial vectors (because this promoter should be inserted into shuttle plasmids or virus plasmids).


Source

This promoter is synthesized de novo using DNA synthesis techniques.

References