Composite

Part:BBa_K3868095:Design

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)


pCas


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1572
    Illegal EcoRI site found at 2469
    Illegal EcoRI site found at 3978
    Illegal PstI site found at 3473
    Illegal PstI site found at 3720
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1572
    Illegal EcoRI site found at 2469
    Illegal EcoRI site found at 3978
    Illegal NheI site found at 1331
    Illegal PstI site found at 3473
    Illegal PstI site found at 3720
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1572
    Illegal EcoRI site found at 2469
    Illegal EcoRI site found at 3978
    Illegal BamHI site found at 2408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1572
    Illegal EcoRI site found at 2469
    Illegal EcoRI site found at 3978
    Illegal PstI site found at 3473
    Illegal PstI site found at 3720
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1572
    Illegal EcoRI site found at 2469
    Illegal EcoRI site found at 3978
    Illegal PstI site found at 3473
    Illegal PstI site found at 3720
    Illegal AgeI site found at 3900
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Gene is from S. pyogenes for the pCas plasmid of the CRISPR-Cas9 system.


References