Coding
Part:BBa_K3867001:Design
Designed by: Lei Huang Group: iGEM21_ICJFLS (2021-10-13)
ADG1 (ADP glucose pyrophosphorylase 1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 256
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 413
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use two restriction enzymes EcoRI and PstI to insert the ADG1 gene into the vector, so these two enzyme sits are avoided.
Source
It comes from the rDNA sequence of Arabidopsis thaliana.