Translational_Unit

Part:BBa_K3866038

Designed by: Pericles Vasileiou   Group: iGEM21_Thessaly   (2021-10-15)


J23115:sfGFP:Terminator

Usage and Biology

This TU includes the sfGFP gene placed under the control of the constitutive Anderson promoter J23115 BBa_J23115.

Design Notes

The coding sequence was domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo.

Figure 1. The level α module of the sfGFP Translational Unit: α1: J23115:RBS-sfGFP-Double terminator

Verification of Cloning

Figure 2.: (C=Cut, U=Uncut) Restriction digestion of α-sfGFP TU (samples 3,4,5) with: HindIII, Expected bands: 2847bp, 801bp, 146bp, Positive result: C5

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 192
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None