Composite

Part:BBa_K3861025

Designed by: Kristin Funke   Group: iGEM21_Humboldt_Berlin   (2021-10-01)


PlldR-sicP-sptP-Pep8-HiBiT

Composite part consisting of BBa_K3861000 and BBa_K3861012. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI-1 T3SS. The HiBit tagged Pep8 allows to track the Pep8 in translocation assays.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
    Illegal PstI site found at 519
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None