Composite

Part:BBa_K3855606

Designed by: Sebastian Bayer   Group: iGEM21_BOKU-Vienna   (2021-10-18)


Sucrose-6-phosphat hydrolase sacA Expression casette for L. plantarum with an inducable Promotor and

Sucrose-6-phosphat hydrolase sacA featuring a N-terminal secretion signal for lactobacilli and a C-terminal 6* His-tag followed by 2 stop codons.

Expression casette for L.plantarum with an inducable promotor. Enzyme features a His-Tag and a secretion signal."


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 200
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 562
    Illegal SpeI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1564
    Illegal BamHI site found at 1245
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 200
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 200
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None