Composite

Part:BBa_K3848010

Designed by: Atharv Salvi   Group: iGEM21_IISER_Bhopal   (2021-10-19)


TRAIL-Smac fusion peptide secretion cassette.

This cassette has the required components to generate an anti-tumor fusion protein in the combined conditions of hypoxia and high lactate. The promoter responsible for the regulation of transcription is the AND gate (Part:BBa_K3848002, see the part page for more information) and the anti-tumor fusion peptide is the TRAIL-Smac fusion construct (BBa_K3848000, see the part page for more information). The product of this cassette is designed to be secreted out of the bacterial cell as TRAIL is ligand for the TRAIL receptor on cancer cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 47
    Illegal BamHI site found at 898
    Illegal BamHI site found at 904
    Illegal XhoI site found at 440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1018
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1039


[edit]
Categories
//cds/ligand
//chassis/prokaryote/ecoli
//classic/signalling/sender
//function/cellsignalling
Parameters
chassisE. coli
positive_regulatorshypoxia, lactate (both required for expression)
signalling_moleculesignals (TRAIL receptors) DR4/5 for apoptosis