Plasmid_Backbone

Part:BBa_K3846050:Design

Designed by: Marcel Arvid Zimmeck   Group: iGEM21_Hamburg   (2021-10-12)


modified pAM5411 (phytobrick acceptor backbone)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1
    Illegal XbaI site found at 8957
    Illegal PstI site found at 8342
    Illegal PstI site found at 8900
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 6855
    Illegal PstI site found at 8342
    Illegal PstI site found at 8900
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1
    Illegal XbaI site found at 8957
    Illegal PstI site found at 8342
    Illegal PstI site found at 8900
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1
    Illegal XbaI site found at 8957
    Illegal PstI site found at 8342
    Illegal PstI site found at 8900
    Illegal NgoMIV site found at 5909
    Illegal NgoMIV site found at 5919
    Illegal NgoMIV site found at 6898
    Illegal NgoMIV site found at 7040
    Illegal AgeI site found at 4412
    Illegal AgeI site found at 4813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 8065
    Illegal BsaI.rc site found at 8943


Design Notes

Source

https://www.addgene.org/132663/ (modified)

References